PRIMER PICKING RESULTS FOR pair_1

Using mispriming library primer_repeat_boundary2_lib
Using internal oligo mishyb library primer_repeat_boundary2_lib
Using 0-based sequence positions
OLIGO            start  len      tm     gc%   any    3'   rep seq
LEFT PRIMER          0   20   91.85  100.00 20.00 20.00  0.00 GCGCGCGCGCGCGCGCGCGC
RIGHT PRIMER       181   20   91.74  100.00 14.00 14.00  0.00 GCGCGCGCGCGCGCCGCGCG
INTERNAL OLIGO     111   20   91.85  100.00 20.00 20.00  0.00 GCGCGCGCGCGCGCGCGCGC
SEQUENCE SIZE: 182
INCLUDED REGION SIZE: 182

PRODUCT SIZE: 182, PAIR ANY COMPL: 17.00, PAIR 3' COMPL: 17.00

    0 GCGCGCGCGCGCGCGCGCGCTATATATATATATATATATATATATATATATTATATATAT
      >>>>>>>>>>>>>>>>>>>>                                        

   60 ATATATATATATTATATATATATATATATATATTAATATATATATATATATGCGCGCGCG
                                                         ^^^^^^^^^

  120 CGCGCGCGCGCATATATATATATATATATATATATATTATAACGCGCGGCGCGCGCGCGC
      ^^^^^^^^^^^                               <<<<<<<<<<<<<<<<<<

  180 GC
      <<

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer
^^^^^^ internal oligo


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left      83     0     0     0     0     0     0     0     0     0     0    82     0     0     1
Right     83     0     0     0     0     0     0     0     0     0     0    71     0     0    12
Intl     163     0     0     0     0     0     0     0     0     0     0   121     0     0    42
Pair Stats:
considered 381, unacceptable product size 369, no internal oligo 11, ok 1
primer3 release 2.0.0


PRIMER PICKING RESULTS FOR pair_0

Using mispriming library primer_repeat_boundary2_lib
Using internal oligo mishyb library primer_repeat_boundary2_lib
Using 0-based sequence positions
NO PRIMERS FOUND

SEQUENCE SIZE: 163
INCLUDED REGION SIZE: 163


    0 TATATATATATATATATATATATATATATATATATATATATATATATATATATATATATA
   60 TATATATATATTATATATATATATATATATATATATATATATATATATATATATATATAT
  120 ATATATATATATATATATATATATATATATATATATATATATA


Statistics
         con   too    in    in   not          no    tm    tm  high  high  high        high      
         sid  many   tar  excl    ok   bad    GC   too   too   any    3'   lib  poly   end      
        ered    Ns   get   reg   reg   GC% clamp   low  high compl compl   sim     X  stab    ok
Left      64     0     0     0     0     0     0     0     0     0     0    64     0     0     0
Right     64     0     0     0     0     0     0     0     0     0     0     0     0     0    64
Intl     144     0     0     0     0     0     0     0     0     0     0     0     0     0   144
Pair Stats:
considered 32, unacceptable product size 32, ok 0
primer3 release 2.0.0


